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電話：025-86880024
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聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP61130COLO-320人結腸腺癌細胞的詳細資料：
COLO-320人結腸腺癌細胞
| CBP61130 | |
| I. General information | |
| Synonyms: | COLO-320 |
| Background: | established from the tumor mass of a 55-year-old Caucasian woman with a moderately undifferentiated adenocarcinoma of the sigmoid colon in 1977; cells were described in the literature to produce several hormones, e.g. serotonin, epinephrine; cells express MYC mRNA |
| Species: | human Homo sapiens |
| Tissue: | colon |
| Disease: | colon adenocarcinoma |
| Gender: | N/A |
| Morphology: | undifferentiated, round cells in suspension and loosely adherent in monolayers; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 38 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, G6PD, MDH, NP |
| Cytogenetics: | human hyperdiploid karyotype - 5350-54<2n>XX, -3, -3, +5, +7, +12, -13, -17, +18, +22, +6mar, t1;5 p11;q11, der5t5;?p15;?, der7t7;?q22;?, der9t9;?p11;?, del9p22, del12p12, i13q, der14t14;?q24;?, der18t18;?p11;? - variable numbers of supernumerary dmins were present in 90% of cells |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer 4008-750-250. |