公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60985PA317小鼠逆轉錄病毒包裝的胚胎成纖維細胞的詳細資料：
PA317小鼠逆轉錄病毒包裝的胚胎成纖維細胞
| CBP60985 | |
| I. General information | |
| Synonyms: | PA317 |
| Background: | The PA317 cell line was derived from TK- NIH/3T3 cells by cotransfection with packaging construct DNA pPAM3 carried in pBR322 and the herpes simplex virus thymidine kinase TK gene carried in pBR322. |
| Species: | Mus musculus, mouse |
| Tissue: | embryo |
| Gender: | embryo |
| Morphology: | fibroblast |
| Growth Mode: | adherent |
| Culture Medium: | DMEM+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | Cells shipped from the ATCC have been selected in medium containing 0.03 mM hypoxanthine, 0.001 mM amethopterin methotrexate, and 0.02 mM thymidine prior to freezing, and should be grown in HT medium for 4 days after receipt. After this, the cells are stable for at least 1 month in the absence of further selection. Introduction of retroviral vectors into these cells, by infection or by transfection, results in production of retrovirus virions with an amphotropic host range that are capable of infecting cells of many mammalian species. The percentage of PA317 cells that are capable of packaging retroviral vectors decreases slowly with continued passaging of the cell line, presumably due to the loss of the transfected DNA used to create the line. Brief selection about 5 days in medium containing 0.03 mM hypoxanthine, 0.001 mM amethopterin methotrexate, and 0.02 mM thymidine will select for cells that retain the packaging function. After selection, the cells should be grown in HT medium 0.03 mM hypoxanthine, 0.02 mM thymidine for 4 days to dilute any residual amethopterin. For more information, please contact Cobioer 4008-750-250. |