公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60862Lec1倉鼠卵巢細胞的詳細資料：
Lec1倉鼠卵巢細胞
| CBP60862 | |
| I. General information | |
| Synonyms: | Lec1 |
| Background: | This line is a mutant clone derived from the parental CHO clone Pro-5 a proline auxotroph, see ATCC CRL-1781 by selection for resistance to wheat germ agglutinin. |
| Species: | Cricetulus griseus, hamster, Chinese |
| Tissue: | ovary |
| Gender: | female |
| Morphology: | epithelial |
| Growth Mode: | loosely adherent |
| Culture Medium: | Alpha-MEM ribonucleosides and deoxyribonucleosides+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | The cells lack GlcNAc glycosyl transferase so that N-linked carbohydrates are blocked at the Man5-GlcNAC2-Asn intermediate. The line may be useful for studying the effects of altered N-linked glycosylation on the function and compartmentalization of endogenous glycoproteins or glycoproteins introduced by viral infection or transfection of foreign DNA. The population contains Pro+ revertants at low frequency approximately 1 in 100,000 cells, and should be suitable for complementation studies which require the Pro- marker. The cells are proline auxotrophs and must be grown in a medium that contains proline 40 mg/L. The population contains Pro+ revertants at low frequency approximately 1 in 100000 cells, and should be suitable for complementation studies which require the Pro- marker. For more information, please contact Cobioer 4008-750-250. |