公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60791M-07e人巨細胞白血病細胞的詳細資料：
M-07e人巨細胞白血病細胞
| CBP60791 | |
| I. General information | |
| Synonyms: | M-07e |
| Background: | established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia AML M7 at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-?, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent within 3-4 weeks, presumably due to outgrowth of independent cells |
| Species: | Homo sapiens, human |
| Tissue: | operipheral blod |
| Disease: | acute megakaryoblastic leukemia |
| Gender: | famale,6-month-old |
| Morphology: | acute megakaryoblastic leukemia |
| Growth Mode: | single round cells in suspension, a few cells are lightly adheren |
| Doubling Time: | 40 hours |
| Culture Medium: | RPMI-1640 + 20% FBS + GM-CSF 10 ng/ml or 10% vol conditioned medium of cell line 5637 We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - Immunology: CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR Cytogenetics: human near-diploid karyotype - 4645-46<2n>XX, t11;21p11;p13, add13p13, add22p13 For more information, please contact Cobioer 4008-750-250. |