公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60721HEL人紅白血病細胞的詳細資料：
HEL人紅白血病細胞
| CBP60721 | |
| I. General information | |
| Synonyms: | HEL |
| Background: | established from the peripheral blood of a 30-year-old man with erythroleukemia AML M6 in relapse after treatment for Hodgkin lymphoma in 1980; cells were described in the literature to be capable of spontaneous and induced globin synthesis and to carry the JAK2 V617F mutation |
| Species: | human Homo sapiens |
| Tissue: | N/A |
| Disease: | erythroleukemia |
| Gender: | N/A |
| Morphology: | round, large to occasionally giant polynucleated, single cells in suspension; few cells adherent; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 36 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: | MEM+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Ciprobay ciprofloxacin, then negative in DAPI, microbiological culture, PCR assays |
| Immunology: | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, CD41a +, CD71 +, CD235a +; |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH, NP |
| Cytogenetics: | human hypotriploid karyotype with 2.3% polyploidy - 6360-64<3n>XYY, -2, -9, -10, -10, -11, -14, -16, -16, -17, -19, +20, +21, +2mar, del2q32, t3;6p13;q16, der5t5;17q10;q10, der6t1;6p13;p21, der7add7p14;q32, add8p21, der9t9;??;11p24;??;q13, del11q13, add15p11, del20q13, r20p11q11, dup21q11q22.3-qter, psu dic22;9t9;??;22p24;??;p11-13 - resembles published karyotype - carries also masked 5q- and 20q- consistent with AML M6/MDS |
| Molec. Genetics: | expression of mutated JAK2 confirmed by RT-PCR and sequencing |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer 4008-750-250. |