公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60658KYSE-520人食管鱗癌細胞的詳細資料：
KYSE-520人食管鱗癌細胞
| CBP60658 | |
| I. General information | |
| Synonyms: | KYSE-520 |
| Background: | established from the moderately differentiated invasive eosphageal squamous cell carcinoma resected from lower intra-thoracic esophagus of a 58-year-old Japanese woman prior to treatment tumor had invaded the adventitia; described as carrying p53 mutation and amplification of the oncogene MYC |
| Species: | human Homo sapiens |
| Tissue: | esophageal |
| Disease: | esophageal squamous cell carcinoma |
| Gender: | N/A |
| Morphology: | epitheloid cells growing adherently as monolayers, occasional giant cells present ca. 1%; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 30 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | originally confirmed as human with IEF of AST, LDH, MDH and later by STR profiling |
| Cytogenetics: | human flat-moded hypotriploid karyotype with 8% polyploidy - 58-60<3n>XX, -X, +1, +1, -2, -2, -4, -5, -6, +7, -8, -10, -10, -11, -12, -12, -13, -14, -15, -16, -17, -18, +20, +20, -21, -21, -22, -2, +7-8mar, addXp11, addXq11, add1q11, der1del1q12t1;7p36;q21, der1del1q12t1;5p36;q11, del1q21, del1p34, add2q37, del3q13, add4p11, add4p15, add5q11x1-2, add6q1?, t6;15p10;q10x1-2, del7q11x1-2, add7p22, add9p23, der11add11p15t11;12q24;q11, add11p15, add16q2?3, add17q24, add18p11, add19p13del22q13 |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer 4008-750-250. |