公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60629Karpas 422人非霍奇金淋巴瘤的詳細資料：
Karpas 422人非霍奇金淋巴瘤
| CBP60629 | |
| I. General information | |
| Synonyms: | KARPAS 422 |
| Background: | Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma intra-abdominal, diffuse large cell lymphoma, refractory, terminal. Carries t14;18 IGH-BCL2 fusion gene break point in major breakpoint region, MBR. |
| Species: | human Homo sapiens |
| Tissue: | B cell lymphoma |
| Disease: | Human B cell non-Hodgkin lymphoma |
| Gender: | 73 year-old female |
| Morphology: | Round polygonal cells growing singly or in small clusters in suspension |
| Growth Mode: | Suspension |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 11 D13S317: 9,13 D16S539: 14 D5S818: 11,12 D7S820: 11,13 THO1: 8,9.3 TPOX: 8,9 vWA: 14,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+20%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Hyperdiploid with 10% polyploidy |
| Subculture Routine: | If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube; slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%.Maintain cultures between 5 - 20 x100,000 cells/ml by splitting saturated cultures 1:2 every 2-4 days; 5% CO2; 37°C. Doubling time approximately 60-90 hours. Round polygonal cells growing singly or in small clusters in suspension. |
| Comments: | For more information, please contact Cobioer 4008-750-250. |