公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60496OE33人食管癌細胞的詳細資料：
OE33人食管癌細胞
| CBP60496 | |
| I. General information | |
| Synonyms: | OE33 |
| Background: | The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus Barrett's metaplasia of a 73 year old female patient. The tumour was identified as pathological stage IIA UICC and showed poor differentiation. OE33 express HLA-A, -B and -C antigens MHC class I and ICAM-1 constitutively. Expression of HLA-DR MHC class II can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice. Cultures derived from ECACC stocks of this cell line have been whole genome sequenced Contino et al 2016 confirming the presence of many of the known mutations that drive oesophageal cancer |
| Species: | human Homo sapiens |
| Tissue: | oesophagus |
| Disease: | 73 year old female |
| Gender: | Human Caucasian oesophageal carcinoma |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 10 D13S317: 14 D16S539: 12 D5S818: 11 D7S820: 9,10 THO1: 7,8 TPOX: 8,11 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Aneuploid |
| Subculture Routine: | Split sub-confluent cultures 70-80% 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days i.e. replacing half the old medium with fresh. |
| Comments: | For more information, please contact Cobioer 4008-750-250. |