公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60495OE19人食管癌細胞的詳細資料：
OE19人食管癌細胞
| CBP60495 | |
| I. General information | |
| Synonyms: | OE19 |
| Background: | The cell line OE19, also known as JROECL19, was established in 1993 from an adenocarcinoma of gastric cardia/oesophageal gastric junction of a 72 year old male patient. The tumour was identified as pathological stage III UICC and showed moderate differentiation. The cell line OE19 expresses HLA-A, -B and -C antigens MHC class I constitutively. Treatment with interferon-gamma induced the expression of ICAM-1 CD54. Expression of HLA-DR MHC class II on interferon-gamma addition was only measured in a sub-population of OE19. The cells express epithelial cytokeratins and are tumourigenic in nude mice.The Y chromosome could not be detected in this cell line by short tandem repeat STR-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. |
| Species: | human Homo sapiens |
| Tissue: | Oesophagus/gastric cardia |
| Disease: | Human Caucasian oesophageal carcinoma |
| Gender: | N/A |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 11,13 D13S317: 9,11 D16S539: 12,13 D5S818: 11,14 D7S820: 8 THO1: 8,9 TPOX: 8 vWA: 16,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Aneuploid |
| Subculture Routine: | Split sub-confluent cultures 70-80% 1:8 i.e. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.These cells grow slowly in islands and when resuscitated from frozen it can take up to 7 days to reach 70% confluence. |
| Comments: | For more information, please contact Cobioer 4008-750-250. |