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地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
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聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP6047523132/87人胃癌細胞的詳細資料：
23132/87人胃癌細胞
| CBP60475 | |
| I. General information | |
| Synonyms: | 23132/87 |
| Background: | established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987 |
| Species: | human Homo sapiens |
| Tissue: | gastric |
| Disease: | gastric adenocarcinoma |
| Gender: | N/A |
| Morphology: | adherent epithelial cells growing as confluent monolayers; |
| Growth Mode: | N/A |
| Doubling Time: | about 50 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Ciprobay ciprofloxacin, then negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -; initial data published by the originators showed negativity, we found the cells to express cytokeratin-7 |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH |
| Cytogenetics: | human hypertetraploid karyotype with 12% polyploidy - 4745-52<2n>XY, +20, t1;15p11;p11, t6;12 p21;q21, i13q, t13;14p10;q10 - closely resembles published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer 4008-750-250. |