公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60363EFM-19人乳腺癌細胞的詳細資料：
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| CBP60363 | |
| I. General information | |
| Synonyms: | EFM-19 |
| Background: | established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma ductal type, histological grading II in 1979 |
| Species: | human Homo sapiens |
| Tissue: | breast |
| Disease: | breast carcinoma |
| Gender: | N/A |
| Morphology: | epitheloid, growing in clusters at first, then as monolayer; |
| Growth Mode: | N/A |
| Doubling Time: | 50-60 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin - |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH |
| Cytogenetics: | human highly rearranged hypotriploid karyotype with 5.8% polyploidy - 6258-65<3n>X, derX, apparent ch 17 nullisomy and preponderance of marker chromosomes, e.g. add1p11, idel1q11q?22, i1p, i2p, add4p11, add4p11, etc. - resembes original description |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer 4008-750-250. |