公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP60240L-363人多發性骨髓瘤細胞的詳細資料：
L-363人多發性骨髓瘤細胞 CBP60240 | |
| I. General information | |
| Synonyms: | L-363 |
| Background: | established from the peripheral blood of a 36-year-old woman with plasma cell leukemia IgG in 1977; cells were described in the literature to be EBNA-negative and to express mRNA for proto-oncogene BCL2; plasma cell leukemia is related to multiple myeloma |
| Species: | human Homo sapiens |
| Tissue: | N/A |
| Disease: | plasma cell leukemia |
| Gender: | N/A |
| Morphology: | single, large, round or oval cells in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 25 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Ciprobay ciprofloxacin, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, LDH, PEP B |
| Cytogenetics: | human hyperdiploid karyotype with 11% polyploidy - 4945-50<2n>X, -X, -X, -6, +7, +8, +11, +11, +14, +19, -22, t5;8q12/13;q24.32, der8t5;8q12/13;q24.32, der8t7;8q11.2;p22, del11q13x2, add14q32, del17p12, add19q13 - sideline with add13q34 - 11q13 and 14q32 breakpoints recurrent in PCL - resembles published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer 4008-750-250. |