Background:

Established from the tumor tissue of a 15-year-old Argentine boy with well differentiated hepatocellular carcinoma in 1975; cell line is patented; cells were described to not harbor a hepatitis B virus genome; cells reportedly produce a variety of proteins: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichymotrypsin, haptoglobin, ceruloplasmin, plasminogen, complement C3, C4, C3 activator, fibrinogen, alpha1-acid glycoprotein, alpha2-HS glycoprotein, ?-lipoprotein, retinol binding protein. They have been grown successfully in large scale cultivation systems. The cells will respond to stimulation with human growth hormone.

Species:

Homo sapiens, human

Tissue:

Liver

Disease:

Hepatocellular carcinoma

Gender:

Male, 15 years, Caucasian

Morphology:

Epithelial-like cells growing as monolayers and in small aggregates

Growth Mode:

Adherent

Doubling Time:

50~60hrs

DNA Profile:

Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10
THO1: 9
TPOX: 8,9
vWA: 17
Cobioer’s Cell Line Authentication Service

Culture Medium:

MEM + 10% FBS+ 1% Non Essential Amino Acids NEAA + 1mM Sodium Pyruvate NaP
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% Complete Medium+10%DMSO

Photo:

Receptor Expression:

Insulin; insulin-like growth factor II IGF II

Genes Expressed:

Alpha-fetoprotein alpha fetoprotein; albumin; alpha2 macroglobulin alpha-2-macroglobulin; alpha1 antitrypsin alpha-1-antitrypsin; transferrin; alpha1 antichymotrypsin; alpha-1-antichymotrypsin; haptoglobin; ceruloplasmin; plasminogen;,complement C4; C3 activator; fibrinogen; alpha1 acid glycoprotein alpha-1 acid glycoprotein; alpha2 HS glycoprotein alpha-2-HS-glycoprotein; beta lipoprotein beta-lipoprotein; retinol binding protein retinol-binding protein

Cellular Products:

Cellular products: alpha-fetoprotein alpha fetoprotein; albumin; alpha2 macroglobulin alpha-2-macroglobulin; alpha1 antitrypsin alpha-1-antitrypsin; transferrin; alpha1 antichymotrypsin; alpha-1-antichymotrypsin; haptoglobin; ceruloplasmin; plasminogen;

Effects:

No, in immunosuppressed mice

Immunology:

Cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -

Comments:

The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities.
The cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone oxidative stress.

There is no evidence of a Hepatitis B virus genome in this cell line.

Misidentified: Originally thought to be a hepatocellular carcinoma cell line but shown to be from an hepatoblastoma.

For more information, please contact Cobioer 4008-750-250.