Background:

Established from the primary colon carcinoma of an adult man; cells were described to carry a RAS mutation in codon 13 and to be tumorgenic in nude mice

Species:

Homo sapiens, human

Tissue:

Colon

Disease:

Colorectal Carcinoma

Gender:

Male, adult

Morphology:

Epithelial-like small cells growing as monolayer

Growth Mode:

Adherent

Doubling Time:

25~48hrs

DNA Profile:

Amelogenin: X,Y/X, X
CSF1PO: 7,10/7,9,10,11
D13S317: 10,12
D16S539: 11,13/11,12,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9/8,8
TPOX: 8,9/8,8

Culture Medium:

McCoy's 5a +10%FBS
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% Complete Medium+10%DMSO

Photo:

Genes Expressed:

Carcinoembryonic antigen (CEA) 1 ng per 10e6 cells per 10 days.

Tumor Formation:

Yes, in nude mice

Immunology:

cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -

Comments:

The cells are positive for keratin by immunoperoxidase staining.
HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression.

For more information, please contact Cobioer (4008-750-250).