Background:

Established from the primary colon tumor (adenocarcinoma) of a 72-year-old Caucasian man in 1974. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.

Species:

Homo sapiens, human

Tissue:

Colon

Disease:

Colorectal adenocarcinoma

Gender:

Male, 72 years adult, Caucasian

Morphology:

Epithelial adherent cells; after splitting cells start to grow in colonies

Growth Mode:

Adherent

Doubling Time:

65~80hrs

DNA Profile:

Amelogenin: X
CSF1PO: 11
D13S317: 11,13,14
D16S539: 12,13
D5S818: 12,13
D7S820: 11,12/12, 12
THO1: 6
TPOX: 9,11

vWA: 16,18
Cobioer’s Cell Line Authentication Service

Culture Medium:

MEM+20%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% Complete Medium+10%DMSO

Photo:

Receptor Expression:

This cell line expressed heat stable enterotoxin (Sta, E. coli) and epidermal growth factor (EGF).

Genes Expressed:

Keratin,retinoic acid binding protein 1,retinol binding protein 2

Cellular Products:

keratin
retinoic acid binding protein 1

Tumor Formation:

Yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with colonic primary (grade II)

Immunology:

Cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -

Virus Susceptibility:

Human immunodeficiency virus 1

Comments:

Upon reaching confluence, the cells express characteristics of enterocytic differentiation [PubMed: 1939345]. Caco-2 cells express retinoic acid binding protein I and retinol binding protein II [PubMed: 9040537]

For more information, please contact Cobioer (4008-750-250).