公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP74109hIL15 Effector Reporter Cell的詳細資料：
hIL15 Effector Reporter Cell
| CBP74109 | |
| I. Background | |
| IL-15 has been shown to be produced by human fetal astrocytes and microglial cells in response to IL-1beta, IFN-gamma, or TNF- alpha and may thus play a role in T-cell mediated immune responses in the human central nervous system. IL-15 stimulates proliferation of the established T-cell line CTLL-2, CD8(+) memory T- cells require IL-15 for proliferation. IL-15 is also able to induce generation of cytolytic cells and LAK cells activity in vitro. IL-15 appears to function as a specific maturation factor for NK-cells and can mimic the bone marrow microenvironment in vitro, which is required for NK- cells to acquire lytic functions. IL-15 induces proliferation of mast cells in the absence of functional IL-2 receptor components and utilizes a receptor of 60-65 kDa that is distinct from known components of T-cell IL-15 receptors. The IL-15 receptor on mast cells recruits JAK2 and STAT5, instead of JAK1, JAK3, and STAT3 and STAT5 that are activated in T-cells. The alpha subunit of the IL-15 receptor (IL-15RA) is structurally related to the alpha subunit of the IL-2 receptor and confers high affinity binding of IL-15 to its receptor. IL-15 binds to the beta and gamma chains of the IL2 receptor, which are known to be required for ligand internalization and signal transduction. | |
| II. Introduction | |
| Expressed gene: | hIL15 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | hIL15 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+10ng/ml IL-3+600ug/ml hygromycin+2ug/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Representative Data | |
Figure 1. Dose Response of Recombinant Human IL15 in Human IL15 Effector Reporter Cells，the EC50 was 1.2ng/ml. | |
