公司名稱：南京康佰生物科技有限公司
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
地址:南京市雨花區長虹路222號
郵編：
電話：025-86880024
手機: 18066071954
聯系人: 蔣
傳真：
E-mail: duhaitao@cobioer.com
CBP74029LIGHT/CHO 細胞的詳細資料：
| CBP74029 | |
| I. Background | |
| LIGHT (TNFSF14) is a key component of the communication system that controls the responses of T-Cells. LIGHT activates two cell surface receptors, the Herpesvirus Entry Mediator (HVEM) and the Lymphotoxin-beta Receptor (LTβR). The LIGHT-HVEM axis is an important costimulatory pathway for T cells, it has been shown to activate NF-κB; LIGHT/HVEM pair are co-stimulatory immune checkpoint molecules for cancer immunotherapy, LIGHT expressing cells have been shown to be more potent than the soluble LIGHT for activating HVEM expressed on T cell surface. | |
| II. Description | |
| Recombinant stable clonal CHO cell line constitutively expressing full length human LIGHT protein, also known as TNFSF14, (Genbank #NM_003807). Surface expression is confirmed by flow cytometry. | |
| III. Introduction | |
| Host Cell: | CHO, Chinese Hamster Ovary |
| Expressed gene: | Light |
| Stability: | 32 passages |
| Synonym(s): | TNFSF14, light cho |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+300ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | This cell line is useful for LIGHT binding assays, flow cytometry, or for screening for LIGHT antibodies. Also useful for immune checkpoint co-stimulatory assay when co-cultured with HVEM/NF-κB-luciferase/Jurkat reporter cell line. |
| IV. Description of Host Cell Line | |
| Organism: | Cricetulus griseus, hamster, Chinese |
| Tissue: | Ovary |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure. Recombinant stable clonal CHO cell line constitutively expressing full length human LIGHT protein, also known as TNFSF14, (Genbank #NM_003807). Surface expression is confirmed by flow cytometry. | |