Cell Line Name：

Host Cell:

BA/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

 RPMI-1640+10%FBS+2ug/ml puromycin

Mycoplasma Status:

Negative

NTRK1 rearrangements involve the kinase domain of the NTRK1 gene which includes 17 exons located on chromosome 1q21-22 (Alberti et al. 2003). The frequency of NTRK1 fusions in patients with adenocarcinoma histology is 3.3% of cases (3 out of 91 patients; Vaishnavi et al. 2013).
a In preclinical studies, cell lines expressing MPRIP-NTRK1 or CD74-NTRK1 were inhibited by ARRY-470, a TRKA/B/C inhibitor (Vaishnavi et al. 2013).
b In preclinical studies, cell lines expressing MPRIP-NTRK1 or CD74-NTRK1 were inhibited by lestaurtinib, a FLT3/TRKA inhibitor (Vaishnavi et al. 2013).
c A patient with non-small cell lung cancer harboring an MPRIP-NTRK1 fusion mutation was treated with crizotinib, which has activity against TRKA in addition to ALK, MET and ROS1 (Vaishnavi et al. 2013). The patient demonstrated a small radiographic decrease in tumor size but experienced disease progression after three months (Vaishnavi et al. 2013)

1. WB of  TPM3-NTRK1-G667C/BaF3 expression

2. Anti-proliferation assay

Figure 2. SNP of  TPM3-NTRK1-G667C/BaF3

Figure 3. Sanger Sequencing of  TPM3-NTRK1-G667C/BaF3

Figure 4. CTG Proliferation Assay of BaF3 TPM3-NTRK1 G667C Cells (C1).