Background:

Established from a metastatic lymph node of a 69-year-old man with colon carcinoma in 1984

Species:

Homo sapiens, human

Tissue:

Colon

Disease:

Colorectal adenocarcinoma, derived from metastatic site: Lymph node.

Gender:

Male, 69 years

Morphology:

Round or spindle-shaped adherent cells growing as monolayer

Growth Mode:

Adherent

Doubling Time:

60~70hrs

DNA Profile:

Amelogenin: X
CSF1PO: 12, 12
D13S317: 10, 13
D16S539: 9, 9
D5S818: 13, 14
D7S820: 8, 12
THO1: 9, 9.3
TPOX: 8, 8
vWA: 16, 19
Cobioer’s Cell Line Authentication Service

Culture Medium:

RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% Complete Medium+10%DMSO

Photo:

NA

Comments:

Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin –
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV -