Background:

The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line by limiting dilution; the clone was selected on the basis of morphological homogeneity and exclusive apical villin localization; C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon; isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II ; the cells express substantial levels of BB myosin I similar to that of the human enterocyte; although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.

Species:

Homo sapiens, human

Tissue:

Colon

Disease:

Colorectal adenocarcinoma

Gender:

Male, 72 years, Caucasian

Morphology:

Epithelial

Growth Mode:

Adherent

Doubling Time:

NA

DNA Profile:

Amelogenin: X
CSF1PO: 11,11
D13S317: 11, 13, 14/8,11,13,14
D16S539: 12, 13
D5S818: 12, 13
D7S820: 11, 12
THO1: 6,6
TPOX: 9, 11
vWA: 16, 18
Cobioer’s Cell Line Authentication Service

Culture Medium:

MEM+20%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+ 0.01 mg/ml human transferrin
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% Complete Medium+10%DMSO

Photo:

Comments:

C2BBe1 cells are sensitive to any imbalance in the media pH. If you are using a DMEM medium formulation containing 3.7 g/L sodium bicarbonate, C2BBe1 cells must be incubated in a 95% air; 10% carbon dioxide (CO2) environment . The increased CO2 is necessary when using 3.7 g/L bicarbonate in order for the medium to be buffered properly, or these cells will not attach well. If you use the recommended ATCC DMEM medium (Catalog No. 30-2002) which contains only 1.5 g/L sodium bicarbonate, this medium is formulated for use with 5% CO2. The basal medium should be supplemented with 10% non heat inactivated Fetal Bovine Serum (FBS) (ATCC® 30-2020™) and 0.01 mg/mL human transferrin (Sigma T-5391). Any floating cells may be viable and should be retained by gentle centrifugation and added back to the adherent population at the time of medium renewal or subculture.

For more information, please contact Cobioer (4008-750-250).